Illumina MiSeq sequencing; qiita_ptid_2124:10564.TNSJJS0H - SRA

ERX1757916: Illumina MiSeq sequencing; qiita_ptid_2124:10564.TNSJJS0H
1 ILLUMINA (Illumina MiSeq) run: 333,122 spots, 100.3M bases, 45.4Mb downloads

Design: Baseline microbiota of hematopoietic stem cell transplant donors and recipients

Submitted by: University of California San Diego Microbiome Initiative (University of California San Diego Microbiome Init)

Study: Baseline Fecal Samples of Hemapoetic Stem Cell Transplant Donors and Patients

PRJEB16057 • ERP017899 • All experiments • All runs

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We evaluated whether the baseline intestinal microbiota (16S-rRNA) of hematopoietic stem cell transplant patients and donors are implicated in transplant outcomes--namely, acute gastrointestinal graft-versus-host disease (GVHD).

Sample: Baseline Fecal Samples of Hemapoetic Stem Cell Transplant Donors and Patients; 10564.TNSJJS0H

SAMEA4506995 • ERS1406174 • All experiments • All runs

Organism: human gut metagenome

Library:

Name: 10564.TNSJJS0H

Instrument: Illumina MiSeq

Strategy: OTHER

Source: METAGENOMIC

Selection: PCR

Layout: SINGLE

Construction protocol: Extracted DNA was normalized ~10^6 template/microliter, then primers 534F (GTGCCAGCMGCCGCGGTAA) and 806R (GGACTACHVGGGTWTCTAAT) were used for targeted amplification of the V4 region of 16S-rRNA for Illumina paired-end sequencing.

Experiment attributes: (show all 11 attributes...) (hide...)

center_name: Daniel Frank Laboratory, University of Colorado, A (show full text...)(hide...)

Daniel Frank Laboratory, University of Colorado, Anschutz

center_project_name: Stem cell

pcr_primers: FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT

primer: GTGCCAGCMGCCGCGGTAA

run_center: Daniel Frank Laboratory, University of Colorado, A (show full text...)(hide...)

Daniel Frank Laboratory, University of Colorado, Anschutz

run_date: 2015

run_prefix: TNSJJS0H-R1

sample_center: UC-Anschutz

sequencing_meth: sequencing by synthesis

target_gene: 16S rRNA

target_subfragment: V4

Runs: 1 run, 333,122 spots, 100.3M bases, 45.4Mb

Run	# of Spots	# of Bases	Size	Published
ERR1688299	333,122	100.3M	45.4Mb	2016-10-18

ID:: 3300725

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